Theoretically Rosetta are designed to supply to codon bias. If you are working with a codon optimized gene. you can use directly the BL21 cells. BL21(DE3) Competent E.coli: C2527: 6 x 0.2ml: Agilent SURE2 Competent Cells: Agilent : 200152: 10 x 0.10ml: NEB Stable Competent E. coli (High Efficiency) C3040: 20 x 0.05ml: XL1-Blue MRF' Supercompetent Cells: Agilent: 200230: 5 x 0.2 mL: NEB ® Stable Competent E. coli (High Efficiency) C3040: 6 x 0.2 mL: BL21(DE3)pLysS Competent Cells What is the difference between BL21 (DE3) pLysS and BL21 (DE3) pLysE? There are two cell lines that contain the T7 lysozyme; these are called BL21 (DE3) pLysS and BL21 (DE3) pLysE. The following table summarizes some of the key features of the two cell lines. Protein expression level in KRX cells are as high or higher than levels expressed in BL21(DE3)-derived strains. However, pre-induction protein expression levels in Single-Step (KRX) Competent Cells are significantly lower than those of BL21(DE3)-derived strains (Figure 2.2) and therefore recommended for the expression of protein toxic to E. coli. BL21 (DE3) produced the highest amount of ADI protein, followed by Rosetta (DE3). The following activity assay showed that ADI from BL21 (DE3) and Rosetta (DE3) had the most activity. Rosetta™ (DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. - Find MSDS or SDS, a COA, data sheets and more information. .

difference between rosetta and bl21